📘 Formula (Arnon Method)
Total = (20.2 × A645) + (8.02 × A663)
Chl a = (12.7 × A663) − (2.69 × A645)
Chl b = (22.9 × A645) − (4.68 × A663)
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❓ FAQ
What is A645 and A663?
These are absorbance values measured using a spectrophotometer.
Which method is used?
Arnon (1949) method for chlorophyll estimation.